An improved high-quality genome assembly and annotation of Tibetan hulless barley

We reported an improved high-quality assembly of Tibetan hulless barley genome with 4.0Gb in size. We employed the falcon assembly package ,scaffolding and error correction tools to finish improvement using PacBio long reads sequencing technology, with contig and scaffold N50 lengths of 1.563Mb and 4.006Mb, respectively, representing more continuous than the original Tibetan hulless barley genome nearly two orders of magnitude. We also re-annotated the new assembly, and reported 61,303 stringent confident putative protein-coding genes, of which 40,457 is HC genes.