Single molecule sequencing shows an extensive structural diversity of Carbapenemase-producing enterobacteria.

Carbapenemase-producing bacteria are resistant against almost all beta-lactam and cephalosporin class antibiotics and represent a growing health crisis. Carbapenemases reside predominantly in mobile genetic elements and rapidly spread across genetic backgrounds and species boundaries. Here, we report more than one hundred finished Carbapenemase and ESBL producing genomes sequenced using Oxford Nanopore and Illumina technologies. We observed the rapid movement of Carbapenemases between distinct genomic neighborhoods, sequence types, and species boundaries using this diverse collection of high quality closed genomes and their associated plasmids.