Effects of Mosquito Saliva on Human PBMCs

Mosquito saliva is thought to have immunomodulatory effects on human immune cells. To test this, we stimulated human PBMCs (from two separate human donors) with mosquito saliva obtained from Aedes aegypti mosquitoes and performed flow cytometry to determine changes in immune cell populations at day 5 post stimulation. Media stimulation was used as a negative control and pokeweed mitogen and LPS were used as positive controls.
Notes:
Data were acquired on a LSRII Fortessa using HTS attachment. Data were analyzed using flow jo v10. Compensation was performed in Flowjo. Single color controls using ultra e-comp beads for voltage checks and compensation. Fluorescence minus one controls using PBMCs for gating controls.