Single cell RNA-sequencing on E17.5 mouse lungs and tracheas

To identify clear mesenchymal cell clusters, sequencing libraries of embryonic lung cells were prepared using the 10x Genomics Chromium system. After quality control samples were integrated. The cells were visualized in two dimensions according to their gene expression profiles using Uniform Manifold Approximation and Projection (UMAP). We FACS sorted live cells from three E17.5 murine lungs and tracheas. Library preparation and single cell RNA-seq was performed on purified single cells.为了识别清晰可辨的间充质细胞簇，研究人员采用10x Genomics Chromium系统制备了胚胎肺细胞的测序文库。质控完成后对样本进行整合。研究人员基于细胞的基因表达谱，采用均匀流形近似与投影（Uniform Manifold Approximation and Projection, UMAP）将细胞可视化至二维空间。我们从3份E17.5阶段的小鼠肺及气管中，通过荧光激活细胞分选（Fluorescence-Activated Cell Sorting, FACS）分离得到活细胞。研究人员对纯化后的单细胞完成了文库制备与单细胞RNA测序（single cell RNA-seq）。