Srf is essential for mesodermal cell migration during elongation of the embryonic body axis

Timecourse analysis of SRF dependent genes in the nascent mesoderm of mouse embryos. Srf was conditionally deleted by a T::cre driver and the three embryonic stages analyzed represent embryos before onset of the phenotype (E8.5) and progressive appearance of the phenotype (E8.75 and E9.0). Total RNA obtained from dissected caudal ends of SRF(flex/flex); T:cre and control littermate embryos at three different stages.小鼠胚胎新生中胚层内SRF（Serum Response Factor）依赖型基因的时序表达分析。本研究通过T::cre驱动子条件性敲除Srf基因，本次分析涵盖三个胚胎发育阶段：分别为表型出现前的E8.5，以及表型逐步显现的E8.75与E9.0。实验所用总RNA提取自三个不同发育阶段的SRF(flex/flex); T:cre小鼠及其同窝对照胚胎的剖离尾端组织。