Human helicase RECQL5-Flag ChIP DNA from Saccharomyces cerevisiae strain W303-1AR5, asynchronous culture

We expressed human RECQL5 in the yeast model Sacharomyces cerevisiae. Being RECQL5 the only member of the family shown to interact with the RNA polymerase II we explored the recruitment of RECQL5 to chromatin. Data reveal a genome wide recruitment of RECQL5 at actively transcribed regions that increases toward the 3’ end of the gene. ChIP-chip studies were performed with antibodies against Flag-tagged RECQL5 protein in yeast wild-type cells expressing human RECQL5