Time-resolved transcriptomics of S. cerevisiae and S. pastorianus in response to plasma membrane stresses

Yeasts are beneficial microorganisms for human society and are utilized for academic and industrial purposes. For academic purposes, S. cerevisiae is a well-investigated model for studying eukaryotic cellular processes. For industrial purposes, S. pastorianus, which has a hybrid genome of S. cerevisiae and S. eubayanus, has been served for lager beer production. During fermentation, S. pastorianus produces ~7% of EtOH, which induces plasma membrane (PM)/cell wall stress in yeast. Therefore, S. pastorianus may experience PM stress and adapt to the self-forming environment during fermentation. However, how yeast adapts to PM stress remains unclear. Here, we investigated the temporal cellular responses of S. cerevisiae and S. pastorianus during adaptation to PM stresses by time-resolved mRNA-seq analysis. Our data showed different transcriptional phenotypes between S. cerevisiae and S. pastorianus during adaptation. The results may reflect the distinct nature of the two yeasts that have evolved in different nutritional environments. The dataset presented here would provide a promising resource for studying the characteristic nature of these differentially domesticated yeasts upon PM stresses.酵母是对人类社会具有重要价值的有益微生物，广泛应用于学术研究与工业生产领域。在学术研究层面，酿酒酵母（S. cerevisiae）是研究真核细胞生命过程的经典模式生物；工业应用中，拥有酿酒酵母（S. cerevisiae）与欧陆酵母（S. eubayanus）杂交基因组的巴氏酵母（S. pastorianus），被广泛用于拉格啤酒的工业化生产。在发酵过程中，巴氏酵母会产生约7%的乙醇（EtOH），该物质会对酵母造成质膜（plasma membrane, PM）与细胞壁胁迫。因此，巴氏酵母在发酵过程中会面临质膜胁迫，并需适应自身形成的发酵环境。然而，酵母如何适应质膜胁迫的分子机制目前仍不明确。本研究通过时间分辨率转录组测序（time-resolved mRNA-seq）技术，分析了酿酒酵母（S. cerevisiae）与巴氏酵母（S. pastorianus）在适应质膜胁迫过程中的时序细胞应答反应。研究数据显示，两种酵母在适应胁迫过程中呈现出截然不同的转录表型。该结果或反映了两类酵母在不同营养环境中演化形成的独特生物学特性。本数据集可为研究经差异化驯化的两类酵母在质膜胁迫下的特性提供极具价值的科研资源。