Climbing behavior of 60 day-old flies.

Healthy looking 60 day-old male flies were selected for this assay. Ten flies were placed in a 100 ml glass cylinder. Following a brief and gentle tapping, the distribution of flies was then counted in 3 bins at different heights (above or at 35 mm height, between 7.5 mm and 35 mm, and 7.5 mm or lower). Climbing pattern of flies expressing SOD1 in motoneurons (A), glial cells (B), and co-expressing SOD1 in both motoneurons and glial cells (C) are shown. Flies expressing human SOD1 proteins in glial cells show a significant shift of distribution to the bottom of the cylinder compared to M1B>UAS-dsod1 (the control fly) and in comparison with their expression in motoneurons (A) or dual expression in MNs + glia (C). These observations suggest a significant impairment of climbing activities at 60 days in flies expressing human SOD1s in glia. The statistical significances were calculated using Prizm software, Two-way ANOVA and * p

本研究选取了60日龄的健康雄性果蝇作为实验对象。将10只果蝇置于100毫升的玻璃圆柱形容器中。经过短暂而轻柔的敲击后，于不同高度（高于或等于35毫米处、7.5毫米至35毫米之间，以及7.5毫米以下）的三个区域对果蝇的分布进行了计数。展示了表达SOD1蛋白的神经元（A）、胶质细胞（B）以及同时在神经元和胶质细胞中共同表达SOD1的果蝇（C）的攀爬模式。在胶质细胞中表达人类SOD1蛋白的果蝇，相较于M1B>UAS-dsod1（对照组果蝇）及其在神经元中的表达（A）或神经元与胶质细胞的双重表达（C），显示出向圆柱底部分布的显著偏移。这些观察结果表明，在胶质细胞中表达人类SOD1蛋白的果蝇在60日龄时其攀爬活动能力受到显著损害。统计显著性使用Prizm软件、双因素方差分析以及* p