The effects on the deficient of GDH1 on gene expression in LN229 cells.

Glutamine is an important nutriment for cancer cell growth that provides biological sources for nucleic acid and fatty acid synthesis, but the role of glutaminolysis in signal transduction and glioblastoma (GBM) progression remains little known. Here, we investigated whether GDH1, a key glutamate dehydrogenase, affects the expression levels of signal transduction factors by mRNA expression profiling of wild-type and GDH1 knockdown cells of the glioblastoma cell line LN229 cells Overall design: Divide Ln229 cells into two groups: one group is wild-type cells, and the other group is subjected to GDH1 gene knockdown. Three biological replicates are set for each group for cultivation treatment. Collect cell precipitates and extract RNA for transcriptome sequencing analysis.