Riemerella anatipestifer Transcriptome or Gene expression

The standard strain (ATCC11845) and the induced strain were scraped from the plate into TSB liquid medium, the OD was adjusted to 1, and cultured in TSB liquid medium for 2 hours, and the bacteria were collected. The total RNA was extracted with the bacterial total RNA Extraction Kit [Tiangen biochemical technology (Beijing) Co., Ltd.], and the RNA purity was determined with nano drop 2000, and then sent to Lianchuan biological company for quality inspection. After passing the quality inspection, the transcriptome was detected. The raw data of transcriptome sequencing was filtered to obtain high-quality data information; Deseq v1.20.0 software was used to study the differentially expressed genes among the three groups. The conditions were set as the difference multiple |log2FC|>1, with significance P<0.05. The mRNA of the three samples were screened for differentially expressed genes.