LRP8 is an entry receptor for tick-borne encephalitis viruses

This dataset contains the raw data supporting all main and supplementary figures used in the manuscript "LRP8 is an entry receptor for tick-borne encephalitis viruses" published in PNAS. In this study, we expressed 13 different family members on the surface of cells and demonstrated that LRP8 promotes infection of tick-borne encephalitis viruses (TBEV). Validation studies confirmed direct binding of TBEV and LRP8, and a key role for LRP8 in neuronal cell infection. Our findings also enabled the generation of an inhibitory LRP8-Fc decoy molecule that blocks TBEV infection.

Dataset description

Figure 1: Flow cytometry analysis and infection assays demonstrating the role of LRP8 in promoting TBEV and other tick-borne orthoflavivirus infections in various cell lines.
Filename: Source_Data_Figure_1.xlsx: 11 data tabs and 1 data dictionary tab

Figure 2: Flow cytometry analysis demonstrating that the ligand binding domain is essential for LRP8-mediated TBEV infection in K562 cells.
Filename: Source_Data_Figure_2.xlsx: 2 data tabs and 1 data dictionary tab

Figure 3: Flow cytometry and biochemical assays demonstrating that LRP8 facilitates TBEV attachment and internalization and directly binds to the virus, as shown by qRT-PCR, ELISA, and biolayer interferometry.
Filename: Source_Data_Figure_3.xlsx: 5 data tabs and 1 data dictionary tab

Figure 4: Flow cytometry and plaque assays showing that LRP8-Fc decoy proteins neutralize TBEV infection in various cell lines and significantly reduce virus titers in infected cells.
Filename: Source_Data_Figure_4.xlsx: 4 data tabs and 1 data dictionary tab.

Figure 5: Immunofluorescence and confocal microscopy demonstrating that LRP8 enhances TBEV infection in primary mouse neuronal cells, with significant differences observed between wild-type, Lrp8+/−, and Lrp8−/− neurons.
Filename: Source_Data_Figure_5.xlsx: 2 data tabs and 1 data dictionary tab.

Figure S5: Flow cytometry analysis revealing that LRP8-Fc decoy proteins neutralize infection by TBEV RVP (Himalaya and 886-84 strains) in SH-SY5Y and N2a cells.
Filename: Source_Data_Figure_S5.xlsx: 2 data tab and 1 data dictionary tab.