MicroED datasets from native nanocrystalline granulovirus polyhedrin recorded with a Falcon III camera

The Cydia pomonella crystalline occlusion bodies were provided by Dominik Oberthür (CFEL).

Data collection

Diffraction data sets were collected for 31 crystals (gv_09 does not exist), following this scheme:

Images were collected on a 300 kV Krios system (Thermo Fisher Scientific), using a Falcon III camera. The condenser system was used in nanoprobe mode, making a parallel beam of size 0.6 µm. A camera length of 3.1 m was chosen, providing 2.1 Å resolution at the edge of the detector

The camera was used in linear mode, and with dose protector disabled, using 1s integration time. Data was unbinned

The gain was determined to be ~10 counts/pe (gain for single frame is 390, for 39 frames averaged the gain is 10)

The stage had a tilt speed of 0.5 deg/s

The dose rate was 0.3 el/Å2/s

For each crystal ~10 frames were collected before severe radiation damage

The samples charged slightly, leading to defocused and astigmatic spots in some cases

Data processing

A script for processing data sets from 22 crystals with DIALS version 1.14.4 and CCP4 7.0.073 is provided. This script will need adapting to work with newer versions of the software.