Open chromatin profiling of human mast cells

Here, we used Omni-ATAC-seq to identify open chromatin regions in human peripheral blood-derived MCs and tonsillar MCs Cells were left untreated or sensitized overnight with myeloma-IgE (0.5 μg/ml) and treated with anti-IgE (1 μg/ml) for 2h or 24h. Select samples were pretreated with BAPTA-AM (30 min, 20 μM) or DMSO before anti-IgE treatment. For IL-33-mediated activation, cells were treated with 10 ng/ml recombinant hIL-33.