Small RNA-seq analysis shows reduced tRNA levels in cdk1-as1 mutants. Small RNA-seq analysis shows reduced tRNA levels in cdk1-as1 mutants

Purpose: Determine whether Cdk1 only regulates a specific subset of tDNAs, or whether Cdk1 has a more global impact on tDNA expression; Method: We study the expression of tRNA genes in WT cells and cdk1-as1 mutants during S phase by small-RNAseq; Result: We found that expression of at least 77% of all tDNAs is significantly downregulated in S-phase in the cdk1-as1 mutant compared with the WT strain; Conclussions: Cdk1 promotes transcription of the majority of tRNA genes during the cell cycle. Overall design: Examination of tRNA expression in CDK1 and cdk1-as1 mutant in S phase during the cell cycle