Expression data from A549 human lung adenocarcinoma cells with and without knockdown against CNOT3

The physiological importance of mRNA degradation through the CCR4-NOT deadenylase has recently been highlighted. For example, mutation in CNOT3, a gene coding for sCNOT3 subunit of the CCR4-NOT complex, is found to be associated with T-cell acute lymphoblastic leukemia, T-ALL, though its contribution to other cancers has not been reported. To identify the taget mRNAs of CNOT3 in human non-small cell lung cancer (NSCLC), we performed the microarray analysis using A549 cells with and without knockdown against CNOT3. We established A549 cells stably expressing tetracyclin-induible shRNA against non-targeting control (A549-T-shNTC) or CNOT3 targeting two different sequences (A549-T-shCNOT3-1 or A549-T-shCNOT3-2) using a lentiviral system. We evaluated the mRNA expression of each A549 stable with or without Doxycyclin (DOX; 1 ug/ml) treatment for 72 hours.