Molecular phylogeny and morphological perianth evolution in Corymbia (Myrtaceae), and the implications for generic delimitation: data and tree files

Premise: Eucalypts (Myrtaceae tribe Eucalypteae) are currently placed in seven genera. Traditionally, Eucalyptus was defined by its operculum but when phylogenies placed Angophora, with free sepals and petals, as sister to the operculate bloodwood eucalypts, the latter were segregated into a new genus, Corymbia. Yet generic delimitation in the tribe Eucalypteae remains uncertain. Here we address these problems using phylogenetic analysis with the largest molecular dataset to date.
Methods: We captured 101 low-copy nuclear exons from 392 samples representing 266 species. Our phylogenetic analysis used maximum likelihood (IQtree) and multi-species coalescent (Astral). At two nodes critical to generic delimitation, we tested alternative relationships among Arillastrum, Angophora, Eucalyptus and Corymbia using Shimodaira's AU test. Phylogenetic mapping was used to explore the evolution of perianth traits.
Results: Monophyly of Corymbia relative to Angophora was decisively rejected. All alte..., Sampling—Hereafter, the term \"eucalypts s.l.\" refers to the clade comprising Angophora, Corymbia and Eucalyptus. \"Corymbia s.l.\" refers to the Corymbia clade, comprising C. subg. Corymbia, C. subg. Blakella and Angophora (Fig. 2). Sampling represented all genera within Myrtaceae tribe Eucalypteae sensu Wilson et al. (2005) except Allosyncarpia, Eucalyptopsis and Stockwellia. Within the eucalypts, both subgenera of Corymbia (Parra-O et al., 2009) and six of eight subgenera of Eucalyptus—except Acerosae (E. curtisii) and Alveolata (E. microcorys), both of which are monotypic—were sampled. There were 392 species-level taxa, including eleven outgroups, which were selected based on whole-of-family phylogenies by Wilson et al. (2005) and Thornhill et al. (2012, 2015) and included species of Osbornia and Melaleuca (tribe Melaleuceae), Backhousia (tribe Backhousieae), Tristaniopsis (tribe Kanieae), Syncarpia (tribe Syncarpieae) and Arillastrum (tribe Eucalypteae). The tree was rooted between Me..., , # Perianth Evolution and Implications for Generic Delimitation in the Eucalypts (Myrtaceae): data and tree files

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## Description of files for Dryad:

1. \
Aligned sequences from targeted capture of 101 low-copy nuclear exons from 392 samples representing 329 species-level eucalypt taxa. This is a Phylip-formatted file for input to IQtree for phylogenetic analysis.

2. <101concat-29May.nex.run1.best_scheme.nex>
This file defines 101 boundaries of loci in .phy file, with a separate model for each locus, input to IQtree

3. <101concat-29May.nex.run1.treefile.tre>
Tree from IQtree run1 analysis using data files 1 and 2 with 101 partitions (= individual nuclear loci).

4. <101concat-29May.phy.gz.run2.treefile.tre>
Tree from IQtree run2 analysis using all loci combined into a single partition

5. \
Mesquite dataset with floral traits mapped on IQtree from run2, with a single partition (tree 4 above)