The detection and enumeration of propionibacterium spp from kefir under different storage conditions using pcr and conventional methods

This study aimed to optimize a method to detect Propionibacterium freudenreichii in kefir during refrigerated, frozen, and freeze-dried storage for 28 days. Conventional enumeration was compared with the polymerase chain reaction (PCR) method. For culture-based analysis, serial dilutions were prepared and plated on YEL, PCA, and Pal-Propionic agar. Samples were treated with propidium monoazide before DNA was extracted using the Power Food Microbial DNA Isolation Kit. Propionibacterium freudenreichii was detected using PCR.