Supporting data for “Maternal Rescue of Post-embryonic Neuroblast Migration in C. elegans C-mannosyltransferase Mutants”

The folder contains two files: Dataset 1 is raw data points used to generate figures in my thesis and a dataset containing gene information. Sheet 1 includes measurements of lengths of different C. elegans strains under a range of temperatures as an indication of their dumpy phenotypes. Sheet 2 summarises the neuronal mismigration phenotype in different wild type and mutant strains. Sheets 3-5 details the maternal and paternal effect of a PVM mismigration phenotype across generations in different strains. Sheet 6 measures the maternal effect of dumpiness in dpy-19 strains. Sheets 7 and 8 looks at the penetrance and maternal effect related to the migration of other Q neurons, including AQR, PQR, SDQR and SDQL. Sheet 9 further looks at this maternal effect of the same phenotypes in dpy-19 in other genes. Sheets 10 and 11 include the quantification of dpy-19 smFISH signals found in different strains at different stages. Sheet 12 is a summary of all C. elegans genes that encode for a WXXWXXW motif and their function. Sheet 13 is the quantification of maternal effects observed in dpy-19(-) strains when m6A pathways are knocked-down.Dataset 2 is statistical analysis done to generate significance value for several of the figures above. Sheet 1 includes an unpaired t test to compare the penetrance of a PVM mismigration defect in animals derived from wild-type phenotype and defective-phenotype mothers. Sheets 2-5 are one-way ANOVA tests that measure the significance of dumpiness in dpy-19 strains when a maternal effect is in action.

该文件夹包含两个文件：数据集1包含用于生成论文中图表的原始数据点以及包含基因信息的数据集。工作表1展示了在不同温度下不同C. elegans菌株的长度测量，以此作为其肥胖表型的指示。工作表2总结了不同野生型和突变型菌株中的神经元错位迁移表型。工作表3至5详细描述了不同菌株中PVM错位迁移表型的母性和父性效应跨代传递。工作表6测量了dpy-19菌株中肥胖的母性效应。工作表7和8探讨了与AQR、PQR、SDQR和SDQL等其他Q神经元迁移相关的渗透率和母性效应。工作表9进一步研究了dpy-19中相同表型的母性效应在其他基因中的表现。工作表10和11包括了在不同菌株和不同阶段发现的dpy-19 smFISH信号的量化。工作表12总结了编码WXXWXXW基序的C. elegans基因及其功能。工作表13量化了在m6A通路敲低时，dpy-19(-)菌株中观察到的母性效应。数据集2是对上述图表生成显著性值所进行的统计分析。工作表1包括非配对t检验，用于比较源自野生型表型和缺陷型表型母亲的动物中PVM错位迁移缺陷的渗透率。工作表2至5为一元方差分析测试，用于测量在母性效应作用下dpy-19菌株肥胖的显著性。