JC Polyomavirus Infection of Primary Human Renal Epithelial Cells Is Controlled by a Type I IFN-Induced Response

JC polyomavirus (JCPyV) established a persistent infection, but BK polyomavirus (BKPyV) killed the cells in 15 days. To identify the cellular factors responsible for controlling JCPyV infection and promoting viral persistence, we profiled the transcriptomes of JCPyV- and BKPyV-infected cells at several time points postinfection. We found that interferon-stimulated genes (ISGs) were only activated in the JCPyV and not in the BKPyV-infected cells. Overall design: Primary Human Renal Epithelial cells were either not infected or infected with JC polyomavirus or BK polyomavirus. Three time points were tested: 3 days, 6 days and 9 days post infection. Three independent replicates were analyzed per condition. Total RNA was harvested, and a stranded RNAseq 2x50 bp was performed using Illumina techonology by Beckman Coulter Genomics