Characterization of Glycerophospholipid Molecular Species in Two Species of Arcidaes (<i>Scapharca subcrenata</i> and <i>Scapharca broughtonii</i>)

In this study, the molecular species of glycerophosphocholine (GPCho), glycerophosphoethanolamine (GPEtn), glycerophosphoserine (GPSer), glycerophosphatidylinositol (GPIns), lysoglycerophosphocholine (LGPCho), and lysoglycerophosphoethanolamine (LGPEtn) from arcidaes <i>Scapharca subcrenata</i> and <i>Scapharca broughtonii</i> were characterized using direct infusion-tandem mass (shotgun) method for the first time. At least 201 and 303 glycerophospholipid (GP) molecular species were characterized, respectively. Most of the predominant GP molecular species contained polyunsaturated fatty acids (PUFA), mainly eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA). Meanwhile, the arcidae lipids contained a high proportion of polar lipids (81.57–86.72% of total lipids). Among PL, phosphatidylcholine (30.53–32.04 mol%) and phosphatidylethanolamine (21.48–26.71 mol%) were dominant.本研究首次采用直接进样-串联质谱（shotgun，鸟枪法）法，对魁蛤科的毛蚶（Scapharca subcrenata）与魁蚶（Scapharca broughtonii）中的甘油磷脂酰胆碱（glycerophosphocholine, GPCho）、甘油磷脂酰乙醇胺（glycerophosphoethanolamine, GPEtn）、甘油磷脂酰丝氨酸（glycerophosphoserine, GPSer）、甘油磷脂酰肌醇（glycerophosphatidylinositol, GPIns）、溶血甘油磷脂酰胆碱（lysoglycerophosphocholine, LGPCho）及溶血甘油磷脂酰乙醇胺（lysoglycerophosphoethanolamine, LGPEtn）分子物种进行了表征。 两种贝类样品分别被鉴定出至少201和303种甘油磷脂（glycerophospholipid, GP）分子物种。多数优势甘油磷脂分子物种含有多不饱和脂肪酸（polyunsaturated fatty acids, PUFA），主要为二十碳五烯酸（eicosapentaenoic acid, EPA）与二十二碳六烯酸（docosahexaenoic acid, DHA）。 同时，魁蛤科脂质中的极性脂质占总脂质的81.57%~86.72%，占比极高。在磷脂（phospholipid, PL）组分中，磷脂酰胆碱（phosphatidylcholine，30.53%~32.04 mol%）与磷脂酰乙醇胺（phosphatidylethanolamine，21.48%~26.71 mol%）为优势组分。