Analysis of Intestinal Microbial Diversity and Function Gene Based on Single Cell Amplification Technique

In order to analyze the information of maternal intestinal bacterial diversity and gene function fully and understand the metabolic characteristics or potential of microorganism,the technique combination of single cell amplification and macrogenomics are used in this paper. Total 40 fecal diluent samples belonging to 5 healthy parturients are used for analysis of bacterial community structure and gene function.And given the key role of short-chain fatty acids in energy and immune regulation during pregnancy, the diversity of their biosynthesis-related functional genes and their correlation with core flora were further investigated.