Transcriptomic Changes in Microdissected CCDs after Three Hours Lipopolysaccharide (LPS) Administration in Rats

Ureteral obstruction is marked by disappearance of the vasopressin-dependent water channel aquaporin-2 (AQP2) in the renal collecting duct and polyuria upon reversal. Most studies of unilateral ureteral obstruction (UUO) models have examined late time points, obscuring the early signals that trigger loss of AQP2. Here, we performed RNA-Seq on microdissected rat cortical collecting ducts (CCDs) to identify early signaling pathways post-UUO. To address whether the induction of inflammatory signaling is a cause of loss of AQP2 expression, we directly induced inflammatory signaling in rats with lipopolysaccharide (LPS) administration输尿管梗阻以肾集合管内抗利尿激素依赖性水通道蛋白2（AQP2）消失、梗阻解除后出现多尿为特征。目前针对单侧输尿管梗阻（UUO）模型的多数研究均聚焦于晚期时间节点，这掩盖了触发AQP2缺失的早期信号通路。本研究通过对显微切割的大鼠皮质集合管（CCDs）进行RNA测序（RNA-Seq），以鉴定UUO术后的早期信号通路。为探究炎症信号通路的激活是否为AQP2表达缺失的诱因，我们通过给予大鼠脂多糖（LPS）直接诱导其炎症信号通路激活。