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The goal is to identify the effect of an artificial linkage in the sgRNA on off-target effects relative to IVT sgRNA.
应用场景：
创建时间：
2019-04-09
相关数据集
Cas9 off-target binding to the promoter of bacterial genes leads to silencing and toxicity [RNA-Seq]
Over the last decade, gene-silencing mediated by dCas9 binding to transcribed regions or their promoters, a strategy referred to as CRISPRi, has emerged as a powerful tool in bacterial genetics. While
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dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle
dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle
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Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq. Sequence Requirements for Cascade Function in a Type I-E CRISPR System: RNA-Seq
We measured global RNA levels using RNA-seq in Δcas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers aff
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Validate whether the targeting of crRNAs affected the host cell gene expression
We explored whether the targeting of crRNAs affected the host cell gene expression. To do this, we used a type LNP, termed a lipitoid, to transiently transfect human A549 lung carcinoma cells with the
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Off-target Analysis. Homo sapiens strain:HEK293T
Three potential off-target sites for each gRNA were identified by CRISPOR (http://crispor.org) based on the protospacer adjacent motif (PAM) sequence in the hg38 reference genome and a high mitOfftarg
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