Complete Genome Sequence of Loigolactobacillus coryniformis FOL-19. Complete Genome Sequence of Loigolactobacillus coryniformis FOL-19

Background Loigolactobacillus coryniformis is a member of lactic acid bacteria isolated from various ecological niches. We isolated a novel L. coryniformis strain FOL-19 from artisanal Tulum cheese and performed the whole-genome sequencing for FOL-19 using Illumina NextSeq. Then, genomic characterization of FOL-19 against seven available complete genome sequences of the same species isolated from kimchi, silage, fermented meat, air of cowshed, and dairy was performed. Results The average genome size of 2.86 ±0.06 Mbp, GC content of 42.86% ±0.06, number of CDS of 2835 ±188, number of tRNA of 54.5 ±9, and number of CRISPR elements of 6.87 ±1.53 were achieved. A single Type II-Cas cluster was found in all strains except for meat and cheese isolate. Five strains harbored at least one intact prophage and one strain (i.e., CECT 5711) was predicted to encode Carnocin CP52 bacteriocin gene cluster. Conclusions The presence of CRISPR elements and Cas clusters suggests that L. coryniformis holds a promising potential for being a reservoir for new CRISPR-based tools. These findings put a step forward for the genomic characterization of L. coryniformis strains for biotechnological applications via genome-guided strain selection to identify industrially relevant traits.