Murine uterus expression profile during Peri-implantation: Pre-implantation vs. Post-implantation

Anaylsis differentially expressed genes of mouse peri-implanted uteri comparing pre-implantation uteri (Day2, Day3 and Day4) with post-implantation uteri (Day6, Day7 and Day8) by microassay. This study has built a meaningful basis for future investigation in elucidating the molecular nature of maternal-fetal interactions during pregnancy establishment and maintenance. Pre-implantation uteri VS. Post-implantation uteri. Three biological replicates of each experiments: pre-implantation uteri (Day2, Day3 and Day4): 234, 234(1) , 234(2); 3 mixture of post-implantation uteri (Day6, Day7 and Day8): 678, 678(1) , 678(2). Two hybridizations were performed by using a reverse fluorescence strategy (Cy3, Cy5) for each sample.本数据集通过微阵列实验（microassay）分析小鼠子宫的差异表达基因（differentially expressed genes），比较植入前子宫（第2天、第3天及第4天）与植入后子宫（第6天、第7天及第8天）的基因表达谱差异。本研究为后续阐明妊娠建立与维持过程中母胎交互作用的分子本质奠定了重要基础。实验分为植入前子宫与植入后子宫两组，每组均设置3次生物学重复（biological replicates）：植入前子宫（第2、3、4天）的样本编号依次为234、234(1)、234(2)；植入后子宫（第6、7、8天）的样本编号依次为678、678(1)、678(2)。每个样本均采用反向荧光标记策略（reverse fluorescence strategy），以Cy3与Cy5为标记完成两次杂交实验。