DNA methylation profiles in 3 mutant NPCs and wt NPCs (control, empty vector) at 10 days

To investigate temporal DNA methylation changes occurring during human neural progenitor cells (NPCs) maintenance in later times via genomic DNA methylation measurement by Infinium MethylationEPIC BeadChip assay, we introduced IDH1-R132H, KRAS-G12C, and TP53-R273C mutations separately into NPCs at day 0, with dox induction for 10 days to overexpress the oncogenes. After 10 days of induction, we investigated the DNA methylation profiles in NPCs, in order to investigate the epigenetic change during oncogene induction. We introduced each oncogene into individual wt NPCs, using dox to overexpress the GoF mutation gene for 10 days, then performed an Infinium MethylationEPIC BeadChip assays. Stanford Functional Genomics Facility (SFGF) using the Infinium MethylationEPIC array which interrogates over 850,000 methylation sites at single-nucleotide resolution.