Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution

Nonradiative photothermal (PT) and photoacoustic (PA) processes have found widespread applications in imaging, stimulation, and therapy. Mapping the generation and propagation of PA and PT waves with resolution is important to elucidate how these fields interact with biological systems. To this end, we introduce spatial offset pump-probe imaging (SOPPI). By spatially offsetting the pump beam and the probe beam, SOPPI can image simultaneously PA and PT wave propagation with nanosecond temporal resolution, micrometer spatial resolution, 65 MHz detection bandwidth, and a sensitivity of 9.9 Pa noise equivalent pressure. We first map the PA and PT evolution from a fiber emitter, and how the wave interacts with a mouse skull and brain slices. SOPPI imaging of PA waves from a tapered fiber with water as an absorber shows a wavelength-dependent generation, an evanescent wave-generated PA, and a back-propagated acoustic Mach Cone. At last, a SOPPI-PACT is developed to reconstruct the pigment dis..., , # Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution Dataset DOI: [10.5061/dryad.k3j9kd5kt](10.5061/dryad.k3j9kd5kt) ## Description of the data and file structure The data presented here is for the manuscript titled \"**Spatial offset pump-probe imaging of nonradiative dynamics at optical resolution**\". Data for each figure are organized separately, from Figure1 to Figure5 and SI. **Figure 1. Using a pump probe method to measure the photoacoustic and photothermal field generated by a fiber.** * (**B**). Representative AC signal detected by the pump probe imaging system. *Variable definitions: Â Â Â Â Â Â Â Â Â Â PA: Photoacoustic. Â Â Â Â Â Â Â Â Â Â PT: Photothermal. Â Â Â Â Â Â Â Â Â Â Time: Recording time. Unit: Microsecond. Â Â Â Â Â Â Â Â Â Â AC Intensity: The signal recorded in the AC channel to plot the PA and PT traces. * (**C**). Decomposition of the raw signal. *Variable definitions: Â Â Â Â Â Â Time: Recording time. Unit: Microse...,