Expression profiles of the hippocampus of Rsk2-KO mice

Coffin-Lowry Syndrome (CLS) is a syndromic form of mental retardation caused by loss of function mutations in the X-linked RPS6KA3 gene, which encodes Rsk2, a serine/threonine kinase involved in spatial memory. We analyzed hippocampal gene expression profiles in Rsk2-KO mice to identify changes in molecular pathways. Total RNA was extracted from hippocampi from 6 KO and 6 WT (littermates) 2-month-old male mice. For each genotype, equivalent amounts of RNA from 2 mice were pooled and processed for hybridization to the genome wide oligonucleotide microarray (Murine 430A 2.0 Affymetrix, 22.000 probe sets). Thus, 3 independent pooled samples were hybridized for each genotype. We compared hippocampal gene expression profiles from rsk2-KO and normal littermate mice to identify changes in molecular pathways科芬-劳里综合征（Coffin-Lowry Syndrome, CLS）是一种由X连锁RPS6KA3基因功能丧失性突变引发的综合征性智力障碍，该基因编码Rsk2——一种参与空间记忆过程的丝氨酸/苏氨酸激酶。为鉴定分子通路的改变，我们对Rsk2基因敲除（Rsk2-KO）小鼠的海马基因表达谱开展了分析。本实验使用6只2月龄雄性Rsk2-KO小鼠与6只同窝野生型（WT）雄性小鼠，提取其海马组织总RNA；针对每个基因型，将2只小鼠的等量RNA混合后，进行全基因组寡核苷酸微阵列（Affymetrix Murine 430A 2.0，包含22000个探针组）杂交。每个基因型共设置3个独立混合样本进行杂交。最终，我们通过比较Rsk2-KO小鼠与正常同窝小鼠的海马基因表达谱，以鉴定分子通路的变化。