Loss of IDH1R132H mediated D-2HG production induces genome-wide demethylation in GCIMP low loci in patient-derived glioma cells

Here, we describe and characterize CRISPR-Cas9 based isogenic cell line models using patient-derived IDH1R132H/WT glioma cell lines, and we use these models to explore changes in DNA methylation following genetic deletion of IDH1 alleles that ablate D2HG production. Comparison of CRISPR IDH1 targeted vs. parental cells or unedited clones methylation values.