Quantitative Analysis of Wild Type and Pich-KO fetal liver LSK Transcriptomes

mRNA profiles of embryonic day 14.5 wild-type (WT) and Pich-KO mice were generated by deep sequencing, in triplicate, using Illumina GAIIx. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrow-Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. Quantitative Analysis of Wild Type and Pich-KO fetal liver LSK Transcriptomes本研究通过Illumina GAIIx测序平台，对胚胎第14.5天的野生型（wild-type, WT）及Pich基因敲除（Pich-KO）小鼠开展三次生物学重复的深度测序，以获取其mRNA表达谱。对通过质量过滤的测序读段，我们在转录本异构体水平采用两种分析方案：其一为Burrow-Wheeler Aligner（BWA）结合方差分析（ANOVA），其二为TopHat结合Cufflinks。本数据集聚焦野生型与Pich-KO小鼠胎肝LSK细胞转录组的定量分析。