Development of novel bacteriophage therapy against multidrug-resistant Klebsiella pneumoniae

Antimicrobial resistance is a critical health threat worldwide, with Klebsiella pneumoniae, recognised as a pathogen of critical global priority. This thesis investigates bacteriophage therapy as a potential therapeutic solution against multidrug-resistant K. pneumoniae. New phages were isolated and characterised using a panel of multidrug-resistant isolates from the blood and urine of a patient with recurrent urinary tract infections. The molecular mechanism of phage resistance was examined, and phage-bacterial host interaction was investigated using solvent shift proteomic profiling. This research paves the way for the clinical translation of phage therapy, thereby improving patient outcomes and public health on a global scale.