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Standard Operating Procedures for an in vitro MMR assay

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Standard Operating Procedures (SOP) for nuclear proteins extraction, HEK293T cells transfection, whole cell protein extraction, mismatched plasmid substrate generation and in vitro MMR assay. Protocols were optimized and validated in González-Acosta et al 2019 (title of the manuscript: Validation of an in vitro mismatch repair assay used in the functional characterization of mismatch repair variants).
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2019-09-27
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