Mass spectrometry of excretory/secretory products (ectosomes, exosomes, and soluble proteins) secreted by Toxoplasma gondii tachyzoites isolated from an infected murine model.

Toxoplasma gondii is an intracellular parasite that infects all nucleated cells; to date, this organism is considered as one of the most successful parasites in the world. During infection, internalization, and proliferation the parasite excretes/secretes a high number of proteins, several of them with identified roles, including MIC proteins, ROP proteins and GRA proteins involved in adhesion, invasion and intracellular proliferation respectively. The characterization of the secretome of Toxoplasma gondii opens the possibility to find new molecules for the design of future anti-parasitic strategies. The complete secretome and proteome of the parasite isolated from the murine model as well as their quantification by MS analysis are now available in the present report.