BM trephine - raw DSP count data

Determining the applicability of NanoString GeoMX to the analysis of BM trephine samples. 5 samples were analysed. MDS1, Normal, MDS2, AA1 and AA2. 12 x 300um ROIs were selected in each sample and analysed using GeoMX digital spatial profiling. For MDS1, Normal and MDS2 - ROIs 1-10 are in tissue, ROI 11 in decalcified trabeculum and ROI 12 in blank slide. For AA1 and AA2 - ROIs 1-11 are in tissue and ROI 12 is in blank slide. Raw counts and technical controls (pos and neg ERCC) are shown.

探讨NanoString GeoMX在骨髓穿刺样本分析中的适用性。共分析了5个样本，分别为MDS1、正常、MDS2、AA1和AA2。在每个样本中选取了12个300微米的区域（ROI）进行GeoMX数字空间分析。对于MDS1、正常和MDS2样本，区域1至10位于组织中，区域11位于脱钙的骨小梁中，区域12位于空白载玻片上。对于AA1和AA2样本，区域1至11位于组织中，区域12位于空白载玻片上。展示了原始计数和专业技术控制（阳性及阴性ERCC）。