Genome assembly of 12 Nigrospora species Assembly

To ascertain which genes were influenced in deletion mutants, we newly generated transcriptome data for deletion mutant vs control for each of three convergent genes (ΔNrURED, ΔNrGMF1, and ΔNrGLA) in N. rubi, respectively. After comparing the differentially expressed genes between all three mutants and controls, we found that 868 genes were identified as co-upregulated and 350 genes as co-downregulated (Figs. S7A and S7B). Gene Ontology (GO) enrichment analysis and KEGG pathway analysis revealed that these differentially expressed genes also are mainly involved in the secretory protein synthesis pathway, such as transmembrane transport, hydrolase activity, and protein processing within the endoplasmic reticulum (Figs. S7C and S7D). These results suggest that deletion of the convergent genes affected secretory protein synthesis, modification, and export. transcriptome data for deletion mutant vs control for each of three convergent genes (ΔNrURED, ΔNrGMF1, and ΔNrGLA) in N. rubi