RNAseq profiling of defined immunocyte subsets from human blood, healthy volunteers

In the context of the Human Cell Atlas, the ImmGen consortium generated reference profiles by bulk RNAseq on finely sorted populations of immunocytes from human blood, healthy volunteers. Specific cell populations were sorted by flow cytometry to high purity (2 rounds of sorting, 1,000 cells/sample), as biological duplicates. The cells profiled according to the ImmGen ULI low-input (SmartSeq2) protocol.