Expression data of human endometrial stromal cells after miR-371a-3p mimic transfection

Members of the miR-371 cluster are of the earliest de novo synthesized by embryos and have important actions in mammalian reproduction. MiR-371a is secreted by embryos at the peri-implantation period and its release has been previously associated with poor embryo quality and lower implantation rates in women undergoing IVF. Here we investigated the potential biological role of miR-371a release in regards to embryonic implantation, and specifically the molecular effects that this miRNA exerts on the cells of the endometrial lining. Gene expression changes in endometrial cells upon miR-371a addition were therefore identified using large-scale genomic plattform that covers the whole human genome (Affymetrix). Human endometrial stromal cells were transfected with a miR-371a-3p mimic, to identify transcriptomic changes that might result from miR-371a regulation. Total RNA was extracted, purified, and sent for transcriptomic profliling using the Clariom S human assay (Affymetrix). The genomic data were processed with Partek and online bioinformatics tools to identify any related pathways to implantation.