XLSearch

XLSearch, for identifying cross-linked peptides. Our approach is based on a data-driven scoring scheme that independently estimates the probability of correctly identifying each individual peptide in the cross-link given knowledge of the correct or incorrect identification of the other peptide. These conditional probabilities are subsequently used to estimate the joint posterior probability that both peptides are correctly identified. Using the data from two previous cross-link studies, we show the effectiveness of this scoring scheme, particularly in distinguishing between true identifications and those containing one incorrect peptide.XLSearch：一款用于交联肽鉴定的方法。我们所提出的方法基于数据驱动的评分方案，可在已知另一肽段鉴定结果正确与否的前提下，独立估算每条单肽在该交联反应中被正确鉴定的概率。随后，这些条件概率将被用于计算两条肽段均被正确鉴定的联合后验概率。借助两项先前交联研究的数据集，我们验证了该评分方案的有效性，尤其在区分真实鉴定结果与含单条错误肽的鉴定结果方面表现突出。

搜集汇总

背景与挑战

背景概述

XLSearch是一个用于从串联质谱中识别化学交联肽对的数据库搜索算法。该软件支持搜索和训练两种模式，通过基于数据驱动的评分方案评估肽段正确识别的概率，并输出按联合概率排序的结果。