Mineralocorticoid Receptor Antagonists and Glucocorticoids Differentially Affect Skeletal Muscle Inflammation and Pathology in Muscular Dystrophy

We report the RNA Sequencing of myleoid cells isolated from 4.5-week-old mdx mice that were administered spironolactone, prednisolone, or vehicle for 7 days. We find that both spironolactone and prednisolone downregulated expression of genes involved in inflammation and fibrosis, while prednisolone further perturbed cell cycle genes. Overall design: Single cell suspensions were prepared from quadriceps from 4.5-week-old mdx mice treated with spironolactone, prednisolone, or vehicle for 7 days. Cells from 3 pairs of mdx quadriceps were pooled for each biological replicate. Myeloid cells were isolated and RNA was extracted from myeloid cells.