Seawater carbonate chemistry and environmental DNA and RNA Shedding and Persistence@en

In the present study, we conducted mesocosm experiments to determine the shedding and decay rate constants of eDNA and eRNA in M. gigas (Magallana [Crassostrea] gigas) using mitochondrially encoded tRNA leucine 1 (mt-tl1) marker at various partial pressures of CO2 in seawater. To our knowledge, this is the first study manipulating seawater pH using CO2. We developed a sensitive and specific quantitative PCR-based assay to detect M. gigas eDNA and eRNA. […]