ChIP-chip analysis of Sir2 and Orc1 in Kluyveromyces lactis. Kluyveromyces lactis

Chromatin Immunoprecipitation followed by microarray analysis to identify the location of Sir2 and Orc1 genomic enrichment. Overall design: Sir2-HA was immunoprecipitated from strains LRY2021 or LRY2022. For LRY2021, the IP sample was labeled with Cy5 and the input was labeled with Cy3. For LRY2022, the dyes were swapped. Labeled DNA was hybridized to a custom ChIP-on-Chip 2x105K microarray (Agilent G4498A), designed with 102,839 60-nucleotide probes tiled across the K. lactis genome spaced approximately every 100 bp (AMADID 018357).