RNA-seq analysis of tetrahedral nucleic acid nanomaterials loaded with miR-124 for the treatment of mouse intracerebral hemorrhage

We used collagenase injection into the brain to model intracerebral hemorrhage in mice. Simply put, the mice were immobilized on a stereotaxic frame. 0.05U collagenase VII was injected sequentially into the caudate nucleus (bregma: 0.2 mm anterior, 2.3 mm right lateral, and 3.5 mm deep). Three days after the collagenase injection, we collected brain tissue from normal mice (control group), the hemorrhagic side of the brain from ICH mice (experimental group), and the hemorrhagic side of the brain from ICH mice after intravenous injection of 20 mmol miR124-TNA (intervention group). An intracerebral hemorrhage mouse model was established through collagenase injection, followed by the extraction of total RNA from the peri-hematoma brain tissue. Each group consisted of 3 replicates. A total of 3 groups were formed: the experimental group, the control group, and the intervention group. Finally, high-throughput transcriptome sequencing was performed on the Illumina NovaSeq.