Effect of TDP-43 and FUS silencing on gene expression of inmortalised human myoblast 8220 cell line

To investigate the effects on human muscle precursor cells of a loss of function of Mendelian genes related to familial ALS, such as TARDBP (TDP-43) or FUS, we established human inmortalised myoblast cell lines in which each target gene has been transiently knocked down (KD) by shRNA. Differencial gene expression analysis of RNA-seq data for inmortalised human myoblast 8220 cell line and its KD derivatives (shTDP-43, shFUS). The used plasmids were: SHC001: shRNA Empty Vector Control Plasmid DNA, TRCN000016038: human, TARDBP MISSION shRNA, TRCN0000288639: human, FUS MISSION shRNA, (all from Sigma-Aldrich)).