Vesicular miRNAs as predictive biomarkers for benzo(a)pyrene's exposure

Benzo(a)pyrene is a widespread environmental pollutant associated with the development of toxic effects on human health. This work explores the potential of microRNAs extracted from extracellular vesicles from circulating blood as biomarkers of exposure to this pollutant. 12 independent EVs from PBMC cultures each grown in untreated (DMSO) and 2 µM B[a]P-treated conditions for 48 h, were combined in 3 equal pools (with 4 separate blood donors per pool) for each condition. A volume of 5 µl was used to isolate the small RNA fraction and processed using Qiaseq miRNA library prep kit.