Characterization of a α-L-rhamnosidase from Bacteroides thetaiotaomicron with high catalytic efficiency of epimedin C
收藏国家林业和草原科学数据中心2022-11-03 更新2024-03-06 收录
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资源简介：
In this study, we successfully overexpressed and characterized the α-L-rhamnosidase gene BtRha from Bacteroides thetaiotaomicron VPI-5482 in E. coli. As compared on the enzyme properties, BtRha was very rare to have the highly selective to hydrolyze the α-1,2 and α-1,6 glycoside linkages between rhamnoside and rhamnoside, rhamnoside and glycoside, respectively. And the protein was more efficient and selective in biotransformation epimedin C to icariin. Thus, this study provides a useful α-L-rhamnosidase, which may be used to improve the enzymatic conversion of epimedin C to icariin.
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国家林业和草原科学数据中心
创建时间：
2022-11-03
