Microbiome analysis in bile in patients with obstructive jaundice by 16S rRNA gene amplicon sequencing

Infection often accompanies the bile in obstructive jaundice, but the bacterial infection and its pathogenesis are unknown. Therefore, the aim of our study was to investigate the composition of the bacterial flora in human bile and to determine its relationship to the causative disease, particularly cholangiocarcinoma and pancreatic cancer. Bile was collected from patients with obstructive jaundice. DNA was extracted from the obtained bile. Samples were analyzed using 16S rRNA gene amplicon sequencing. The metabolic function of the microflora will also be analyzed using PICRUSt2. The results of the microflora were compared with clinical parameters to determine the role of bacteria in the pathogenesis.