AICD downregulates cell survival but does not affect differentiation of newly generated hippocampal cells.

A, Experimental design to measure HPC proliferation or HPC maturation. After the last BrdU injection, mice were sacrificed after 1 day (proliferation) or 1 month (maturation) and HPC survival was quantified as the ratio of the number of BrdU+ cells present at 4 weeks post-BrdU to the number of BrdU+ cells present one day after the last BrdU injection. (B) FeCγ25 mice (13.7, SEM 0.02) showed a significant reduction in the cell survival potential compared to wild-type mice (18.3, SEM 0.02) (* p = 0.012, χ2 test). C–D, Quantitative analysis of the number of BrdU+ cells that mature into neurons (C) and astrocytes (D) 4 weeks after birth. Analysis reveals that AICD does not influence the proportion of surviving cells that mature into neurons (NeuN+, wild-type = 54.8, SEM 0.04; FeCγ25 = 56.6, SEM 0.1) or astrocytes (GFAP+, wild-type = 14.0, SEM 0.02; FeCγ25 = 15.1, SEM 0.03). n = 3 for all.