RNAseq analysis of Synph-293 cells

We report the gene expression profile in Synph-293 cells, which are stably FLAG-tagged synphilin-1 overexpressing HEK293 cells. To analyze the changes of gene expression induced by synphilin-1, HEK293 and Synph-293 cells stably FLAG-tagged synphilin-1 overexpressing were lysed, and then, the total RNA was extracted using TruSeq stranded mRNA Sample Preparation Kit (Illumina, CA, USA). The quality of RNA was assessed using an Agilent RNA 6000 Nano kit (Agilent Technologies, CA, USA). Oligo (dT) magnetic beads were used to purify and fragment the mRNAs from 1 μg of total RNA. The fragmented mRNAs were synthesized into double-stranded cDNA. cDNA libraries were purified using Blue Pippin (Sage Science, MA, USA) and amplified using PCR; the quality of these cDNA libraries was assessed using the Agilent High Sensitivity DNA Kit (Agilent Technologies). Subsequent cluster amplification of denatured templates was performed and an Illumina HiSeq2500 sequencer (Illumina) was used to perform sequencing.