Effect of L-NNA, ODQ and potassium channel blockers on ACh-induced relaxation of mesenteric arteries from normal and diabetic rats with or without linagliptin (2 mg/kg oral gavage daily for 4 weeks) treatment in the presence of indomethacin.

A comparison of the sensitivity (pEC50) and maximum relaxation (Rmax) to ACh in the absence (control), or the presence of TRAM-34 (1 μM)
+apamin (1 μM), L-NNA (100 μM)+ODQ (10 μM), L-NNA (100 μM)+ODQ (10 μM)+ TRAM-34 (1 μM) +apamin (1 μM) or L-NNA (100 μM)
+ODQ (10 μM)+TRAM-34 (1 μM) +apamin (1 μM)+Ibtx (100 nM) in endothelium intact mesenteric arteries. All experiments were performed in the presence of indomethacin (10 μM). n = the number of experiments.
a Significantly different to control within each group, P<0.05, Tukey’s test
b Significantly different to normal within inhibitor group, P<0.05, Tukey’s test
c Significantly different to normal+linagliptin within inhibitor group, P<0.05, Tukey’s test
d Significantly different to diabetic within inhibitor group, P<0.05, Tukey’s test. Results are shown as mean±SEM
ND = not determined.
Effect of L-NNA, ODQ and potassium channel blockers on ACh-induced relaxation of mesenteric arteries from normal and diabetic rats with or without linagliptin (2 mg/kg oral gavage daily for 4 weeks) treatment in the presence of indomethacin.