Sequences and molecular properties of peptoids and comparator peptides.

See Figure 1 for the structures of the peptoid monomers indicated in each sequence. HPLC elution is reported as the percentage of acetonitrile in water (% ACN) in the water/acetonitrile (0.1% trifluoroacetic acid) solvent in analytic HPLC. A linear water/acetonitrile (0.1% trifluoroacetic acid) gradient of 5%-95% acetonitrile over 30 min was run on a C18 column. Net charge indicates molecular charges at neutral pH. (“**”, NHis has 10% probability to be charged around neutral pH, so the net charge of 1- NHis1,7 is between +2 to +4). CTLR stands for charge-to-length ratio, which is defined as the ratio of the total number of cationic residues to the total number of residues in each sequence. Peptoids labeled with asterisk have been reported previously to possess antimicrobial activities [23], [24], [25].